New pti quantamaster™ 8000 series fluorometers – horiba a gas has

#########

The industry standard for sensitivity is a signal to noise ratio calculated from a water Raman spectrum. Using this standardized test (see our technical note "SN Determination for PTI QuantaMaster Fluorometer,") our signal to noise ratio is the highest sensitivity in the world, matched only by the Fluorolog3, also from HORIBA. The extreme sensitivity of the PTI QuantaMaster fluorometer is even more remarkable because it is achieved with the lowest wattage lamp in the industry. This remarkable achievement is a result of the intelligent engineering of the unique PowerArc™ arc lamp illuminator featuring an ellipsoidal reflector with the highest possible light gathering efficiency of 67%, and focusing the light in a tight spot at the monochromator slit. As a result, the standard 75W Xe delivers light to the sample more efficiently than higher power lamps featured by other instruments. electricity in the 1920s This reduces energy waste and excessive heat generation by an overpowered light source, not to mention cost, while exceeding the sensitivity of all competitors’ designs. Another contribution to the high sensitivity of the PTI QuantaMaster comes from the asymmetrical, aberration-corrected monochromator, optimized for the best light throughput and stray light rejection.

Suppression of stray light is one of the most critical factors when measuring highly scattering, or low quantum yield samples. Every PTI QuantaMaster series spectrofluorometer is custom made with the highest quality optics to insure the lowest amount of scatter. The standard 300 mm focal length asymmetrical Czerny-Turner monochromators are coma-corrected and individually optimized for purpose as either excitation or emission monochromators, ensuring the lowest amount of stray light contamination for the best detection of the true fluorescence signal. These monochromators boast an impressively high stray light rejection of 1×10-5 in a single excitation monochromator configuration. The excitation and emission monochromators in the QuantaMaster can also by upgraded to double additive 300 mm focal length monochromators, improving the stray light rejection to 1×10-10. gas in back The QuantaMaster also offers an optional order sorting filter wheel for rejection of second order signals from large spectral scans. This ultimate stray light performance was motivated by an increasing demand for photoluminescence spectrometers in materials science, where strongly scattering samples, such as powders, wafers and films, are routinely used. Very low stray light performance will also benefit researchers working in biological, biomedical and environmental areas where cell suspensions, protein and biomembrane solutions, or soil samples, generate high levels of scattered light.

Resolution is of utmost importance to photoluminescence research. High quality resolution can reveal detailed spectral features which are indispensable for applications in materials science and analytical chemistry. Resolution is the key to detecting very narrow lines, which is necessary to study fine interactions in inorganic materials and crystals. The PTI QuantaMaster yields high quality resolution due to innovative optical design, and very minimal optical aberrations.

The PTI QuantaMaster spectrofluorometers use a precision-driven asymmetrical, 300 mm focal length Czerny-Turner monochromator with a triple motorized grating turret and motorized flipping mirrors. More than 30 different gratings are available. Due to the combination of the computer-controlled motor with micro-stepping resolution and available grating selection, it is possible to achieve a minimum 0.022 nm step size. This means that within the UV and VIS spectral regions, you can resolve spectral features well below 0.1 nm.

The PTI QuantaMaster series of modular research grade spectrofluorometers are multidimensional systems for photoluminescence measurements. The foundation of a fluorescence spectroscopy laboratory is built on steady state intensity measurements such as wavelength scans, time-based experiments, synchronous scans and polarization. The PTI QuantaMaster series ensures you get the best possible results for all these measurements with high sensitivity, spectral resolution and stray light rejection. This level of sensitivity is achieved using a unique xenon illuminator, providing safety, cost, and energy consumption benefits not found amongst competitor companies. electricity history facts These conditions make the PTI QuantaMaster system more than capable of meeting the highest demands of research.

The PTI QuantaMaster system is adaptable to every research need, with additions such as TCSPC lifetimes, upconversion lasers and phosphorescence detection up to 5,500 nm. Using the pulsed light source allows for not only spectral and kinetic fluorescence and phosphorescence measurements, but also the measurement of lifetimes in the microsecond to seconds range. This addition is especially beneficial when using fluorescent probes prone to photobleaching, and when characterizing inorganic material with longer lifetimes. The modular design of the PTI QuantaMaster ensures that your system can be easily adapted to your growing research needs.

FRET is a popular technique used to study binding, conformational changes, dissociation and other types of molecular interactions. Applications of FRET are especially common in biomedical research involving protein-protein, protein-nucleic acid interactions, protein folding/unfolding, nucleic acid hybridization, membrane fusion and many others. physics c electricity and magnetism There is also a variety of immunoassays based on FRET. The FRET phenomenon occurs between an excited donor (D) molecule and a ground-state acceptor (A) molecule over a range of distances, typically 10-100 Å. It is a nonradiative process, meaning no photon is emitted or absorbed during the energy exchange. The efficiency of FRET is strongly dependent on the D-A distance and is characterized by the Förster critical radius Ro, a unique parameter for each D-A pair. Once Ro is known, the D-A pair can be used as a molecular ruler to determine the distance, or monitor distance changes between sites labeled by D and A. Since FRET is mostly used to study biological systems, where concentrations are often low and samples can be highly scattering, the PTI QuantaMaster is an ideal fluorometer for this application due to its high sensitivity and excellent stray light rejection. h gas l gas unterschied It is also easy to upgrade to a lifetime option, which can be very beneficial for verification of the FRET mechanism. The PTI QuantaMaster series will also help you take advantage of this technology with the built-in PTI FelixGX FRET Calculator.

Sample temperature plays a critical role in all types of luminescence measurements. For example, when the emission anisotropy is measured, the viscosity will change as a function of the temperature affecting the rotational motion of the fluorophore. The temperature control can be critical for fluorescence quantum yield determination, or any quantitative intensity measurements since the nonradiative deactivation is strongly temperature dependent. Temperature control is essential in fluorescence studies of proteins as it affects thermal stability of proteins, and their folding and unfolding characteristics.

The PTI QuantaMaster series comes standard with a thermostatable cuvette holder where the plumbing is already in place for temperature control utilizing a circulating water bath. If your research requires more precise or extreme temperature control, additional solutions are available, including software controlled Peltier-based variable temperature cuvette holders (single or 4 position) and a liquid nitrogen cryostat. Programmable spectral scans at automatically varying temperatures and temperature ramping experiments are available.

A pulsed light source and the ability to integrate the signal at user-selected time delay are dispensable tools in discriminating spectra based on the lifetime of the respective excited state. Fluorescence emission happens on the picosecond to nanosecond time scale, while phosphorescence occurs on the microsecond to second time scale. By varying the temporal position and the width of the signal detection gate one can selectively detect fluorescence and phosphorescence spectra as attested by phenanthrene spectra on the accompanying figure. Here, the emission of phenanthrene in a frozen glass was measured with gradually increased time delay of the detection gate to diminish contribution of fluorescence. However, the true potential of this technique can be seen in the case of Room Temperature Phosphorescence (RTP) of RNase T1 tryptophan, where the signal was extracted by gating out the overwhelming Trp fluorescence—a task impossible with a continuous excitation source. Conveniently, the same instrument can be used to measure phosphorescence decay of this extremely weak emission by using the Single-Shot Transient Digitizer (SSTD) function of the ASOC-10 interface.

The applications and interest in NIR photoluminescence have been growing rapidly in recent years. gas leak los angeles This trend is spearheaded mostly by extensive research in nanotechnology and materials science. NIR-emitting nanoparticles, lanthanide doped glasses and ceramics used in developing new laser media and photonic devices, single-walled carbon nanotubes, semiconductor and electroluminescent systems are only a few dominant applications. There is also a considerable research effort in the optical fiber communication industry to develop infrared molecular amplifiers for the transmittance window at 1550 nm. In biomedical areas, there is a trend of using NIR-emitting nanoparticles as luminescent markers due to the fact that the light scattering, a notorious problem in UV-VIS fluorescence measurements, is greatly reduced as the wavelength increases. Less interference means better signal to noise with strongly scattering biological samples. NIR light can penetrate tissue at a much greater depth than the UV and VIS— a definite advantage in tissue imaging and therapeutic applications. In photobiology, the detection of singlet oxygen and development of efficient photo sensitizers for PDT has been the dominant application for years. The continuing introduction of new NIR emitters, coupled with better detection and lower cost systems continues to fuel the growth of NIR luminescence applications. PTI offers an extensive line of PTI QuantaMaster NIR photoluminescence systems with a broad range of options and accessories. The detectors offered include both NIR PMTs and solid state photodiodes that span the range of up to 5,500 nm. Most of these detectors can also be used with pulsed light sources, thus providing the lifetime capabilities in NIR. 9gag instagram logo Applications of NIR Fluorescence

The use and demand for optical fiber communication has grown rapidly and applications are numerous, ranging from global networks to desktop computers. There have been three spectral ‘windows’ used for optical transmission: 850 nm, 1310 nm and 1550 nm, with the third window now becoming a globally accepted transmission band. There is a need to insert some light amplifiers along the fiber line. One idea of amplifying the signal is based on a chelated Erbium ion. Erbium belongs to the family of lanthanides and has an emission band in the NIR at about 1550 nm, so it matches perfectly the 3rd optical transmission window. The chelating molecules are excited in the UV or VIS by inexpensive LEDs and transfer the excitation energy by FRET to the Erbium center, thus promoting Erbium to its excited state. Since the energy difference between the excited and ground state of Erbium equals the energy of photons (1550 nm) that are propagated along the fiber, these incoming photons will stimulate the emission from Erbium, enhancing the overall signal.